If you go deeper than that, there's a great deal more to Trigun than any of this.
And theOtaku's Trigun essays are extremely flawed, factually, so I wouldn't trust them at all. IE, Vash and Knives aren't half plant (as in green leafy thing what photosynthesises) They're Plants, short for Power Plant - energy beings that are some form of genetically manipulated human.
In any case, there's more to Trigun religiously than in the Christian sense, although that is the major theme. Wolfwood represents humanity and its search for redemption. Vash is sort of the benevolent god/guardian angel, whereas Knives is a destroying angel. Legato represents sin (especially in the Catholic sense; Pride, Gluttony, and Envy...) and zealotry, of course. Looking at Legato's motivations from a Buddhist perspective is also very...interesting, to say the least! Midvalley, especially in the Manga, is Free Will and survival instinct.
The Princess of Pain has an excellent essay on Trigun v. Christianity on one of her websites, if you can find it.
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